Journal: PLoS ONE

Article Title: Using BD Vacutainer CD4 Stabilization Tubes for Absolute Cluster of Differentiation Type 4 Cell Count Measurement on BD FacsCount and Partec Cyflow Cytometers: A Method Comparison Study from Zimbabwe

doi: 10.1371/journal.pone.0136537

Figure Lengend Snippet: (A) Number of BD Vacutainer CD4 Stabilization Tube samples on BD FacsCount at days 0–30. (B) Number of BD Vacutainer CD4 Stabilization Tube samples on Partec Cyflow at days 0–30.

Article Snippet: The median CD4 count of Partec Cyflow ST samples was 430 cells/μL (IQR 274–588) at day 0 and decreased slightly to 410 cells/μL (IQR 290–526) at day 21 ( part A).

Techniques:

Journal: PLoS ONE

Article Title: Using BD Vacutainer CD4 Stabilization Tubes for Absolute Cluster of Differentiation Type 4 Cell Count Measurement on BD FacsCount and Partec Cyflow Cytometers: A Method Comparison Study from Zimbabwe

doi: 10.1371/journal.pone.0136537

Figure Lengend Snippet: (A) Absolute CD4 cell counts of BD Vacutainer CD4 Stabilization Tube samples on BD FacsCount and Partec Cyflow at days 0–30. (B) Agreement of BD Vacutainer CD4 Stabilization Tube sample results on BD FacsCount at days 0–30 with EDTA sample results on BD FacsCount at day 0. (C) Agreement of BD Vacutainer CD4 Stabilization Tube sample results on Partec Cyflow at days 0–30 with EDTA sample results on BD FacsCount at day 0. (D) Agreement of BD Vacutainer CD4 Stabilization Tube sample results on Partec Cyflow at days 0–30 with same day results of BD Vacutainer CD4 Stabilization Tube sample results on BD FacsCount.

Article Snippet: The median CD4 count of Partec Cyflow ST samples was 430 cells/μL (IQR 274–588) at day 0 and decreased slightly to 410 cells/μL (IQR 290–526) at day 21 ( part A).

Techniques:

Journal: PLoS ONE

Article Title: Using BD Vacutainer CD4 Stabilization Tubes for Absolute Cluster of Differentiation Type 4 Cell Count Measurement on BD FacsCount and Partec Cyflow Cytometers: A Method Comparison Study from Zimbabwe

doi: 10.1371/journal.pone.0136537

Figure Lengend Snippet: Correlation of BD Vacutainer CD4 Stabilization Tube sample results on BD FacsCount and Partec Cyflow over time.

Article Snippet: The median CD4 count of Partec Cyflow ST samples was 430 cells/μL (IQR 274–588) at day 0 and decreased slightly to 410 cells/μL (IQR 290–526) at day 21 ( part A).

Techniques:

Journal: PLoS ONE

Article Title: Using BD Vacutainer CD4 Stabilization Tubes for Absolute Cluster of Differentiation Type 4 Cell Count Measurement on BD FacsCount and Partec Cyflow Cytometers: A Method Comparison Study from Zimbabwe

doi: 10.1371/journal.pone.0136537

Figure Lengend Snippet: Similarity of BD Vacutainer CD4 Stabilization Tube sample results on BD FacsCount and Partec Cyflow over time.

Article Snippet: The median CD4 count of Partec Cyflow ST samples was 430 cells/μL (IQR 274–588) at day 0 and decreased slightly to 410 cells/μL (IQR 290–526) at day 21 ( part A).

Techniques:

Journal: PLoS ONE

Article Title: Using BD Vacutainer CD4 Stabilization Tubes for Absolute Cluster of Differentiation Type 4 Cell Count Measurement on BD FacsCount and Partec Cyflow Cytometers: A Method Comparison Study from Zimbabwe

doi: 10.1371/journal.pone.0136537

Figure Lengend Snippet: Percentage of correctly classified BD Vacutainer CD4 Stabilization Tube sample results on Partec Cyflow for ART eligibility thresholds of 350 and 500 CD4 cells/μL over time with EDTA tube results on FacsCount at day 0 as reference methodology.

Article Snippet: The median CD4 count of Partec Cyflow ST samples was 430 cells/μL (IQR 274–588) at day 0 and decreased slightly to 410 cells/μL (IQR 290–526) at day 21 ( part A).

Techniques:

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Operating characteristics of flow cytometric methods for CD4+ T-cell enumeration, using conventional flow cytometers.

Article Snippet: Cassens 2004 30 , Africa and Asia , ‘Hospital laboratories’ , Various , Partec CyFlow SL (SCC + CD4), NLNW , HIV pos and neg , 434.

Techniques: Cytometry, Flow Cytometry, Cell Counting

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Operating characteristics of Point of Care (POC) technologies for CD4+ T-cell enumeration.

Article Snippet: Cassens 2004 30 , Africa and Asia , ‘Hospital laboratories’ , Various , Partec CyFlow SL (SCC + CD4), NLNW , HIV pos and neg , 434.

Techniques: Fluorescence, Cell Counting

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Characteristics of included studies.

Article Snippet: Cassens 2004 30 , Africa and Asia , ‘Hospital laboratories’ , Various , Partec CyFlow SL (SCC + CD4), NLNW , HIV pos and neg , 434.

Techniques: Flow Cytometry

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Misclassification (%), using a CD4 thresholds of 350 cells/μl and 200 cells/μl.

Article Snippet: Cassens 2004 30 , Africa and Asia , ‘Hospital laboratories’ , Various , Partec CyFlow SL (SCC + CD4), NLNW , HIV pos and neg , 434.

Techniques:

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Operating characteristics of dedicated single platform CD4+ T-cell enumeration systems.

Article Snippet: Cassens 2004 30 , Africa and Asia , ‘Hospital laboratories’ , Various , Partec CyFlow SL (SCC + CD4), NLNW , HIV pos and neg , 434.

Techniques: Flow Cytometry, Staining

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Operating characteristics of manual technologies for CD4+ T-cell enumeration.

Article Snippet: Cassens 2004 30 , Africa and Asia , ‘Hospital laboratories’ , Various , Partec CyFlow SL (SCC + CD4), NLNW , HIV pos and neg , 434.

Techniques: Light Microscopy, Isolation, Microscopy

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Operating characteristics of other commercially available technologies for CD4+ T-cell enumeration.

Article Snippet: Cassens 2004 30 , Africa and Asia , ‘Hospital laboratories’ , Various , Partec CyFlow SL (SCC + CD4), NLNW , HIV pos and neg , 434.

Techniques: Rotator Assay, Cell Isolation, Modification, Software

Journal: Malaria Journal

Article Title: The relationship of Plasmodium falciparum humeral immunity with HIV-1 immunosuppression and treatment efficacy in Zambia

doi: 10.1186/1475-2875-8-258

Figure Lengend Snippet: Characteristics of study population according to HIV-1 status

Article Snippet: CD4 count were performed on all individuals with a direct volumetric absolute CD4 counting instrument (Cyflow ® Counter, Partec, Germany)[ ].

Techniques: Infection, Cell Counting, Variant Assay

Journal: Malaria Journal

Article Title: The relationship of Plasmodium falciparum humeral immunity with HIV-1 immunosuppression and treatment efficacy in Zambia

doi: 10.1186/1475-2875-8-258

Figure Lengend Snippet: Factors Influencing Antibody Levels to Merozoite Blood Stage Antigen within HIV-1 infected Population (N = 115)

Article Snippet: CD4 count were performed on all individuals with a direct volumetric absolute CD4 counting instrument (Cyflow ® Counter, Partec, Germany)[ ].

Techniques: Infection, Cell Counting

Journal: Malaria Journal

Article Title: The relationship of Plasmodium falciparum humeral immunity with HIV-1 immunosuppression and treatment efficacy in Zambia

doi: 10.1186/1475-2875-8-258

Figure Lengend Snippet: Factors Influencing Antibody Levels to Variant Surface Antigen within HIV-1 infected Population (N = 115)

Article Snippet: CD4 count were performed on all individuals with a direct volumetric absolute CD4 counting instrument (Cyflow ® Counter, Partec, Germany)[ ].

Techniques: Variant Assay, Infection, Cell Counting

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Characteristics of included studies.

Article Snippet: , , , DP FC , Partec CyFlow Counter CD4/SCC NLNW , HIV pos , 128 from 121 donors.

Techniques: Flow Cytometry

Journal: Annals of Medical and Health Sciences Research

Article Title: Pattern of CD4 T-lymphocyte Values in Cancer Patients on Cytotoxic Therapy

doi: 10.4103/2141-9248.122054

Figure Lengend Snippet: Variations in day 12 CD4 count with various malignancies

Article Snippet: CD4 lymphocyte count was done using the Partec Cyflow ® 2000 CD4 cell counter, and their socio-demographic data of the patients were assessed using a questionnaire.

Techniques:

Journal: Annals of Medical and Health Sciences Research

Article Title: Pattern of CD4 T-lymphocyte Values in Cancer Patients on Cytotoxic Therapy

doi: 10.4103/2141-9248.122054

Figure Lengend Snippet: Changes in CD4 count of respondents

Article Snippet: CD4 lymphocyte count was done using the Partec Cyflow ® 2000 CD4 cell counter, and their socio-demographic data of the patients were assessed using a questionnaire.

Techniques:

Journal: Annals of Medical and Health Sciences Research

Article Title: Pattern of CD4 T-lymphocyte Values in Cancer Patients on Cytotoxic Therapy

doi: 10.4103/2141-9248.122054

Figure Lengend Snippet: Variations in day 12 CD4 count with various cytotoxic combinations

Article Snippet: CD4 lymphocyte count was done using the Partec Cyflow ® 2000 CD4 cell counter, and their socio-demographic data of the patients were assessed using a questionnaire.

Techniques:

Journal: AIDS Research and Therapy

Article Title: Predictors of first-line antiretroviral therapy failure among adults and adolescents living with HIV/AIDS in a large prevention and treatment program in Nigeria

doi: 10.1186/s12981-020-00317-9

Figure Lengend Snippet: Demographic and clinical characteristics of PLWH at enrollment into the University of Abuja Teaching Hospital HIV Treatment Program

Article Snippet: CD4 count measurement was performed using laser-based CD4 T-lymphocyte enumeration (Cyflow SL, Partec, Munster, Germany).

Techniques:

Journal: AIDS Research and Therapy

Article Title: Predictors of first-line antiretroviral therapy failure among adults and adolescents living with HIV/AIDS in a large prevention and treatment program in Nigeria

doi: 10.1186/s12981-020-00317-9

Figure Lengend Snippet: Univariate and multivariate analysis of predictors of immunologic failure

Article Snippet: CD4 count measurement was performed using laser-based CD4 T-lymphocyte enumeration (Cyflow SL, Partec, Munster, Germany).

Techniques: Cell Counting

Journal: AIDS Research and Therapy

Article Title: Predictors of first-line antiretroviral therapy failure among adults and adolescents living with HIV/AIDS in a large prevention and treatment program in Nigeria

doi: 10.1186/s12981-020-00317-9

Figure Lengend Snippet: Univariate and multivariate analysis of predictors of virological failure

Article Snippet: CD4 count measurement was performed using laser-based CD4 T-lymphocyte enumeration (Cyflow SL, Partec, Munster, Germany).

Techniques: Cell Counting

Journal: PLoS ONE

Article Title: CD4 T-Cell Enumeration in a Field Setting: Evaluation of CyFlow Counter Using the CD4 Easy Count Kit-Dry and Pima CD4 Systems

doi: 10.1371/journal.pone.0075484

Figure Lengend Snippet: Inter-assay precision of FACSCount, CyFlow Counter and Pima CD4.

Article Snippet: Thanks to their practical advantages, Partec CyFlow Counter and/or Alere Pima CD4 systems can be operated easily in resource-limited settings.

Techniques: Inter Assay

Journal: PLoS ONE

Article Title: CD4 T-Cell Enumeration in a Field Setting: Evaluation of CyFlow Counter Using the CD4 Easy Count Kit-Dry and Pima CD4 Systems

doi: 10.1371/journal.pone.0075484

Figure Lengend Snippet: The x-axis represents CD4 counts provided by the FACSCount reference and the y-axis represents the CD4 counts provided by the CyFlow Counter. The solid line represents the regression line, and the dashed line represents the line y = x.

Article Snippet: Thanks to their practical advantages, Partec CyFlow Counter and/or Alere Pima CD4 systems can be operated easily in resource-limited settings.

Techniques:

Journal: PLoS ONE

Article Title: CD4 T-Cell Enumeration in a Field Setting: Evaluation of CyFlow Counter Using the CD4 Easy Count Kit-Dry and Pima CD4 Systems

doi: 10.1371/journal.pone.0075484

Figure Lengend Snippet: The x-axis represents CD4 counts provided by the FACSCount reference and the y-axis represents the CD4 counts provided by the Pima CD4. The solid line represents the regression line, and the dashed line represents the line y = x.

Article Snippet: Thanks to their practical advantages, Partec CyFlow Counter and/or Alere Pima CD4 systems can be operated easily in resource-limited settings.

Techniques:

Journal: PLoS ONE

Article Title: CD4 T-Cell Enumeration in a Field Setting: Evaluation of CyFlow Counter Using the CD4 Easy Count Kit-Dry and Pima CD4 Systems

doi: 10.1371/journal.pone.0075484

Figure Lengend Snippet: Comparison of CD4 T-cell counts between CyFlow Counter and FACSCount.

Article Snippet: Thanks to their practical advantages, Partec CyFlow Counter and/or Alere Pima CD4 systems can be operated easily in resource-limited settings.

Techniques:

Journal: PLoS ONE

Article Title: CD4 T-Cell Enumeration in a Field Setting: Evaluation of CyFlow Counter Using the CD4 Easy Count Kit-Dry and Pima CD4 Systems

doi: 10.1371/journal.pone.0075484

Figure Lengend Snippet: Comparison of CD4 T-cell counts between Pima CD4 and FACSCount.

Article Snippet: Thanks to their practical advantages, Partec CyFlow Counter and/or Alere Pima CD4 systems can be operated easily in resource-limited settings.

Techniques:

Journal: PLoS ONE

Article Title: CD4 T-Cell Enumeration in a Field Setting: Evaluation of CyFlow Counter Using the CD4 Easy Count Kit-Dry and Pima CD4 Systems

doi: 10.1371/journal.pone.0075484

Figure Lengend Snippet: the x-axis represents the average of CD4 count from CyFlow Counter and FACSCount, and the y-axis represents the bias between CyFlow Counter and FACSCount divided by their mean. The solid line represents the mean bias, and the dashed lines represent the upper and lower limits of agreement (LOA = mean bias ±1.96 SD).

Article Snippet: Thanks to their practical advantages, Partec CyFlow Counter and/or Alere Pima CD4 systems can be operated easily in resource-limited settings.

Techniques:

Journal: PLoS ONE

Article Title: CD4 T-Cell Enumeration in a Field Setting: Evaluation of CyFlow Counter Using the CD4 Easy Count Kit-Dry and Pima CD4 Systems

doi: 10.1371/journal.pone.0075484

Figure Lengend Snippet: the x-axis represents the average of CD4 count from Pima CD4 and FACSCount, and the y-axis represents the bias between Pima CD4 and FACSCount divided by their mean. The solid line represents the mean bias, and the dashed lines represent the upper and lower limits of agreement (LOA = mean bias ±1.96 SD).

Article Snippet: Thanks to their practical advantages, Partec CyFlow Counter and/or Alere Pima CD4 systems can be operated easily in resource-limited settings.

Techniques:

Journal: PLoS ONE

Article Title: CD4 T-Cell Enumeration in a Field Setting: Evaluation of CyFlow Counter Using the CD4 Easy Count Kit-Dry and Pima CD4 Systems

doi: 10.1371/journal.pone.0075484

Figure Lengend Snippet: Comparison of CD4 T-cell counts between Pima CD4 and CyFlow Counter.

Article Snippet: Thanks to their practical advantages, Partec CyFlow Counter and/or Alere Pima CD4 systems can be operated easily in resource-limited settings.

Techniques:

Journal: PLoS ONE

Article Title: CD4 T-Cell Enumeration in a Field Setting: Evaluation of CyFlow Counter Using the CD4 Easy Count Kit-Dry and Pima CD4 Systems

doi: 10.1371/journal.pone.0075484

Figure Lengend Snippet: the x-axis represents the CD4 counts from the FACSCount reference; the y-axis represents the relative bias of alternative methods from the FACSCount reference; the solid line represents the zero-bias.

Article Snippet: Thanks to their practical advantages, Partec CyFlow Counter and/or Alere Pima CD4 systems can be operated easily in resource-limited settings.

Techniques:

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Characteristics of included studies.

Article Snippet: Lynen 2006 33 , Cambodia , Laboratory of an NGO-run hospital , BD FACSCount , Partec CyFlow SL_3 CD4+SSC LNW manual gating , ‘Likely to be HIV positive’ , 115.

Techniques: Flow Cytometry

Journal: PLoS ONE

Article Title: CD4 Enumeration Technologies: A Systematic Review of Test Performance for Determining Eligibility for Antiretroviral Therapy

doi: 10.1371/journal.pone.0115019

Figure Lengend Snippet: Operating characteristics of dedicated single platform CD4+ T-cell enumeration systems.

Article Snippet: Lynen 2006 33 , Cambodia , Laboratory of an NGO-run hospital , BD FACSCount , Partec CyFlow SL_3 CD4+SSC LNW manual gating , ‘Likely to be HIV positive’ , 115.

Techniques: Flow Cytometry, Control, Staining, Battery